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Experimental Research on Improving Sperm Motility of Bovine Straw Frozen Semen
Author(s): XU Hongxi, LIU Xiaoning, ZHANG Hongtao, WEI Ximing, SONG Weihong, HAN Zhiqiang, HAN Huansheng, LI Wei, YANG Zuo
Pages: 28-
30
Year: 2011
Issue:
20
Journal: China Dairy Cattle
Keyword: Bovine Straw Frozen Semen; Sperm Motility; Thawing Temperatur;
Abstract: 本文从细管冻精的3个关键技术环节研究了稀释液的种类对精子活力与存活的影响,确定了细管冻精精子在液氮冷冻度过冰晶期对其损伤最小的最佳熏蒸高度(最佳冷冻温度)和最佳熏蒸时间,并且对成品冷冻的细管冻精不同解冻温度及时间进行研究。由柠檬酸钠和果糖组成的稀释液精子存活时间长,Tris稀释液冷冻后精子活力高于其他配方;牛细管冻精最佳熏蒸距离为2cm,时间影响不显著,7~9min均可;用50℃温水15s解冻精子...
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