Effect of the water extract of Macrosolen cochinchinensis(Lour.) Tiegh.leaves on 7,12-dimethylbenz[a]antracene induced female mice liver carcinogensis
Author(s): Adam Hermawan~1, Retno Murwanti~1, Nina Artanti~, Edy Meiyanto~ 1.Cancer Chemoprevention Research Center, Faculty of Pharmacy Universitas Gadjah Mada, Sekip Utara II, Yogyakarta 55281, Indonesia 2.Research Center for Chemistry, Indonesian Institute of Sciences, Kawasan PUSPIPTEK Serpong 15314, Indonesia
Pages: 627-632
Year: 2011 Issue: 6
Journal: Journal of Chinese Pharmaceutical Sciences
Keyword: Macrosolen cochincinensis; Carcinogenesis; Liver cancer; DMBA;
Abstract: Liver cancer is the third most common cause of death from cancer worldwide.Recently,natural products were used widely as an alternative therapy for liver cancer.Previous study reported Macrosolen cochnichinensis(Lour.) Tiegh.that grows in the host star fruit inhibited breast cancer cells growth in vitro.This study aims to observe the effect of water extract of M.cochinchinensis leaves(MCE) on Balb/c mice hepatocyte after initiation of 7,12-dimethylbenz[a]anthracene(DMBA) as a liver cancer model inducer.The experiment consisted of four mice groups,corn oil solvent control group,the DMBA dose 20 mg/kgBW p.o.ten times twice a week,DMBA+MCE dose 250 mg/kgBW,and DMBA+MCE 750 mg/kgBW.Extract which was dissolved into 0.5%CMC-Na was administered daily by the oral route 1 week before,during and terminated 1 week after the DMBA induction.At the end of the study,rat livers were collected and stained with Haematoxyllene and Eosin(H&E) method. Administration of MCE could not inhibit hepatic carcinogenesis in DMBA-induced female mice.There was no difference in liver tissue histopathology profile between the extract treatment group and DMBA control group.
Pages: 627-632
Year: 2011 Issue: 6
Journal: Journal of Chinese Pharmaceutical Sciences
Keyword: Macrosolen cochincinensis; Carcinogenesis; Liver cancer; DMBA;
Abstract: Liver cancer is the third most common cause of death from cancer worldwide.Recently,natural products were used widely as an alternative therapy for liver cancer.Previous study reported Macrosolen cochnichinensis(Lour.) Tiegh.that grows in the host star fruit inhibited breast cancer cells growth in vitro.This study aims to observe the effect of water extract of M.cochinchinensis leaves(MCE) on Balb/c mice hepatocyte after initiation of 7,12-dimethylbenz[a]anthracene(DMBA) as a liver cancer model inducer.The experiment consisted of four mice groups,corn oil solvent control group,the DMBA dose 20 mg/kgBW p.o.ten times twice a week,DMBA+MCE dose 250 mg/kgBW,and DMBA+MCE 750 mg/kgBW.Extract which was dissolved into 0.5%CMC-Na was administered daily by the oral route 1 week before,during and terminated 1 week after the DMBA induction.At the end of the study,rat livers were collected and stained with Haematoxyllene and Eosin(H&E) method. Administration of MCE could not inhibit hepatic carcinogenesis in DMBA-induced female mice.There was no difference in liver tissue histopathology profile between the extract treatment group and DMBA control group.
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