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Purification and determination by high performance liquid chromatography of genipin
Author(s): 
Pages: 233-236
Year: Issue:  1
Journal: Food Science and Technology

Keyword:  京尼平 栀子苷 栀子蓝色素 高效液相色谱;
Abstract: 研究了用β-葡萄糖苷酶水解栀子苷制备京尼平及京尼平的分离纯化方法,同时还建立了京尼平的高效液相色谱检测法。静态吸附实验表明,HPD700对京尼平的吸附率可达96.50%,动态吸附及洗脱实验结果表明,上样流速以1.5BV/h为宜,采用无水乙醇对京尼平的洗脱率最高,达到94.32%。高效液相色谱法对京尼平的分析结果为:京尼平进样量在0.22.0μg范围内,峰面积与进样量呈良好的线性关系,京尼平的回归方程为Y=3.81×106X-19261,相关系数R2为0.9994,方法精密度为1.06%,回收率为98.5%102.2%。结论:本实验制备的京尼平纯度可达98%以上,为制备高端栀子蓝色素创造了条件。
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