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The proliferative effects of angiogenin on human outer root sheath keratinocytes
Author(s): 
Pages: 328-331
Year: Issue:  6
Journal: Journal of Clinical Dermatology

Abstract: 目的:探讨血管生成素对体外培养的人毛囊外毛根鞘角质形成细胞增殖的影响.方法:采用两步酶消化法分离和培养人毛囊外毛根鞘角质形成细胞,将与绿色荧光蛋白基因共表达的血管生成素真核表达质粒pEGFP-ANG转染人毛囊外毛根鞘角质形成细胞,同时设立转染空质粒的pEGFP-C2组和只加转染液的阴性对照组,48 h后利用ELISA法检测细胞上清液中血管生成素的表达,同时四甲基偶氮唑蓝(MTT)法检测细胞增殖.另...
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