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The variation of tryptase expression in the colonic mucosa of patients with diarrhea-predominant irritable bowel syndrome and its effect on the visceral sensitivity
Author(s): 
Pages: 867-871
Year: Issue:  12
Journal: Chinese Journal of Digestion

Keyword:  Irritable bowel syndromeMast cellsTryptasesVisceral sensitivitySubstance PCalcitonin gene-related peptide;
Abstract: 目的 观察腹泻型肠易激综合征(1BS-D)患者结肠黏膜类胰蛋白酶变化及其结肠黏膜孵育上清液对小鼠内脏感觉的影响.方法 IBS-D患者研究:肠镜检查获取24例非感染后IBS-D患者、12名健康志愿者的升结肠黏膜组织4块,采用甲苯胺蓝染色法计数黏膜肥大细胞数量、酶联免疫分析法检测结肠黏膜孵育上清液中类胰蛋白酶浓度.动物实验:56只6~8周龄C57BL/6系雄性小鼠分为7组,分别予健康小鼠升结肠黏膜培养...
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