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Enhanced inhibitory effect and mechanisms of adenovirus-mediated hepatocellular carcinoma supressor gene 1 combined with 5-FU on growth of LoVo cells
Author(s): 
Pages: 90-93
Year: Issue:  2
Journal: Chinese Journal of Digestion

Keyword:  Liver neoplasmsGenestumor suppressorFluorouracilApoptosis;
Abstract: 目的 通过化学治疗药物5-氟尿嘧啶(5-FU)联合重组腺病毒(Ad)肝癌抑制基因-1(HCCS1)感染结直肠癌细胞,观察两者对结直肠癌细胞的联合杀伤作用.并初步探讨相关分子机制.方法 RT-PCR和Western印迹法检测Ad-HCCS1感染结直肠癌LoVo细胞后HCCS1的表达.活细胞计数试剂盒(CCK)-8法测定Ad-HCCS1联合5-FU处理时的细胞存活率.流式细胞仪检测各种处理后细胞凋亡的...
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