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Regulation of insulin-like growth factor I on the expression of stem cell factor in colonic smooth muscle ceils
Author(s): 
Pages: 241-245
Year: Issue:  4
Journal: Chinese Journal of Digestion

Keyword:  Insulin-like growth factor ⅠStem cell factorSmooth muscle cellColonSignal transduction;
Abstract: 目的 探讨激活胰岛素样生长因子(IGF)-I受体后,大鼠结肠平滑肌细胞(SMC)合成干细胞因子(SCF)的变化及其信号途径.方法 酶解法分离培养SD大鼠结肠SMC,免疫组化鉴定.2.5×10~5/ml SMC接种培养,①加入100μg/L IGF-I分别培养0、8、16、24、48 h;②分别加入0、5、10、50、100、150μg/L IGF-I培养16 h.同时设空白对照组和1GF-I组.免...
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