Effect of bromoxynil on membrane potential and respiratory control rate in isolated mitochondria from mice liver and intervention effect of NAC
Author(s): GUAN Xia-fei, ZHAO Guang-ju, CAI qi-qi, WANG Zhi-yi, LU Zhong-qiu, QIU Qiao-meng, HONG Guang-liang, LIANG Huan
Pages: 472-475
Year: 2009 Issue: 8
Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases
Keyword: Nitriles; Acetylicysteine; Mitoehondria liver; Membrane potentials;
Abstract: Objective To demonstrate the effect of bromoxynil on membrane potential and respiratory control rate (RCR) in isolate mitochondria from mice liver tissue in vim and the intervention of NAC. Methods The mitochondrial was randomized to control group, bromoxynil-poisoned group and NAC-prospected group. S3,S4 and RCR of the mitochondria in each sample was detected by the method of oxygen electrode.Each sample was stained by JC-1 and the changes of membrane potential of mitochondria were observed under fluorescence microscope. Results The S3 [(0.031±0.008)nanoatoms oxygen·mg-1·min-1)],RCR (1.820±0.181) of bromoxynil-poisoned group and RCR (4.253±0.210) of NAC-prespected group were significantly lower than those of control group (P<0.01);the S4[(0.017±0.004) nanoatoms oxygen·mg-1·min-1) of NAC-prospected group was significantly higher than control group (P<0.01).The S3[(0.046±0.005) nanoatoms oxygen·mg-1·min-1)] and RCR of NAC-prospected group were significantly higher than group B (P<0.01),S4 [(0.011±0.001) nanoatoms oxygen·mg-1·min-1)] of NAC-prospected group was significantly lower than bromoxynil-poisoned group (P<0.01). Observaion under fluorescence microscope: the red fluorescence of mitochondria was dim or disappearred in bromoxynil-poisoned group while brightened in NAC-prospected group but still dimmer than control group Conclusion In vitro, the mitochondrial RCR and the mitochondrial membrane potential are decreased after the mitochondria is incubated with bromoxynil, and NAC could improve it.
Pages: 472-475
Year: 2009 Issue: 8
Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases
Keyword: Nitriles; Acetylicysteine; Mitoehondria liver; Membrane potentials;
Abstract: Objective To demonstrate the effect of bromoxynil on membrane potential and respiratory control rate (RCR) in isolate mitochondria from mice liver tissue in vim and the intervention of NAC. Methods The mitochondrial was randomized to control group, bromoxynil-poisoned group and NAC-prospected group. S3,S4 and RCR of the mitochondria in each sample was detected by the method of oxygen electrode.Each sample was stained by JC-1 and the changes of membrane potential of mitochondria were observed under fluorescence microscope. Results The S3 [(0.031±0.008)nanoatoms oxygen·mg-1·min-1)],RCR (1.820±0.181) of bromoxynil-poisoned group and RCR (4.253±0.210) of NAC-prespected group were significantly lower than those of control group (P<0.01);the S4[(0.017±0.004) nanoatoms oxygen·mg-1·min-1) of NAC-prospected group was significantly higher than control group (P<0.01).The S3[(0.046±0.005) nanoatoms oxygen·mg-1·min-1)] and RCR of NAC-prospected group were significantly higher than group B (P<0.01),S4 [(0.011±0.001) nanoatoms oxygen·mg-1·min-1)] of NAC-prospected group was significantly lower than bromoxynil-poisoned group (P<0.01). Observaion under fluorescence microscope: the red fluorescence of mitochondria was dim or disappearred in bromoxynil-poisoned group while brightened in NAC-prospected group but still dimmer than control group Conclusion In vitro, the mitochondrial RCR and the mitochondrial membrane potential are decreased after the mitochondria is incubated with bromoxynil, and NAC could improve it.
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