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Study on Continuous Ovum Pick-up from High Producing Dairy Cow and IVF of Oocytes
Author(s): 
Pages: 23
Year: Issue:  1
Journal: China Dairy Cattle

Keyword:  奶牛活体采卵体外受精体外培养;
Abstract: 在超声波扫描仪的指导下,用双孔型采卵针以11.3~12.0kPa抽吸压经阴道对8头高产奶牛分别连续实施7次活体采卵,每4d1次,共采集卵子340个,占可见卵泡数的53.5%.每次头均采集卵子6.1个.卵子经过体外培养、体外受精、体外受精胚体外发育培养,于体外受精后48h、168h统计的卵裂率、囊胚发育率分别为75.0%和29.7%.研究结果表明,在超声波扫描仪指导下对奶牛连续进行活体采卵,将所得卵子进行体外成熟、体外受精、体外培养,在生产中是可行的.
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