Tissue Cultrue and Rapid Propagation of Cymbidium ensifolium with Verge Line Pattern Leaves
Author(s): LI Li, LUO Jun-qin, NIE Zhen-peng, SUN Jian-hua, KE Fu-zhi, XU Jian-guo
Pages: 69-70
Year: 2008 Issue: 2
Journal: SUBTROPICAL PLANT SCIENCE
Keyword: 线艺建兰; 茎尖; 腋芽; 根状茎; 植株再生;
Abstract: 以线艺建兰茎尖、腋芽为外植体,接种在1/2MS + 6-BA 3.0mg/L + NAA 0.3mg/L培养基上形成原球茎,原球茎在1/2MS + 6-BA 0.1mg/L + NAA 1.0mg/L培养基上可大量增殖,并伸长生长形成丛生型根状茎;根状茎在MS + 6-BA 2.0mg/L + PP333 1.0mg/L+ NAA 0.5mg/L培养基上可分化成小苗,分化率达46.3%;小苗在MS + IBA 1.0mg/L +GA 0.5mg/L +香蕉泥100g/L的培养基上生根壮苗,生根率达100%.
Pages: 69-70
Year: 2008 Issue: 2
Journal: SUBTROPICAL PLANT SCIENCE
Keyword: 线艺建兰; 茎尖; 腋芽; 根状茎; 植株再生;
Abstract: 以线艺建兰茎尖、腋芽为外植体,接种在1/2MS + 6-BA 3.0mg/L + NAA 0.3mg/L培养基上形成原球茎,原球茎在1/2MS + 6-BA 0.1mg/L + NAA 1.0mg/L培养基上可大量增殖,并伸长生长形成丛生型根状茎;根状茎在MS + 6-BA 2.0mg/L + PP333 1.0mg/L+ NAA 0.5mg/L培养基上可分化成小苗,分化率达46.3%;小苗在MS + IBA 1.0mg/L +GA 0.5mg/L +香蕉泥100g/L的培养基上生根壮苗,生根率达100%.
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