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Effct on the quality of AFP in HepG-2 cells influenced by isothiocyanates in Broccoli
Author(s): 
Pages: 2-4
Year: Issue:  6
Journal: HARBIN MEDICAL JOURNAL

Keyword:  异硫氰酸盐肝癌细胞甲胎蛋白;
Abstract: 目的 观察异硫氰酸盐(ITCs)对人肝癌细胞生长和细胞内甲胎蛋白变化的影响.方法 以HepG-2为实验对象,分对照组、不同浓度的ITCs组,采用MTT法检测ITCs作用后细胞的生长抑制率,放射免疫法(ELISA)测定细胞中甲胎蛋白(AFP)的含量.结果 异硫氰酸盐能明显抑制HepG-2细胞增殖,细胞形态呈明显的良性分化,甲胎蛋白(alpha-fetopro rein,AFP)合成及分泌量明显下降.结论 西兰花中异硫氰酸盐对HepG-2细胞的增长抑制作用可能与影响甲胎蛋白变化有关.
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