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Improvement of Blood Conservation Condation and DNA Extraction Method from Blood in Dairy Cattle
Author(s): 
Pages: 7-9
Year: Issue:  5
Journal: CHINA DAIRY CATTLE

Keyword:  提取DNA血液基因组DNA中国荷斯坦牛;
Abstract: 本文研究中国荷斯坦牛血液在不同温度、时间保存的条件下(4℃保存0d为A组;-70℃保存30d为B组;4℃保存7d为C组)对DNA提取效果的影响和对比试剂盒法(E)、试剂盒改良法(F、酚-氯仿法(G)提取中国荷斯坦牛血液基因组DNA的效果.试验结果表明:A组与B组差异不显著(P>0.05);A组、B组与C组差异显著(P<0.05).4℃保存0d对DNA提取效果影响最小.在A组中,E与F差异不显著(P>0.05);E、F与G差异显著(P<0.05).综合各方面因素,试剂盒改良法最好.
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