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Preliminary Study on Induction of Hairy Roots in Lonicera macranthoides
Author(s): 
Pages: 55-58
Year: Issue:  1
Journal: NONWOOD FOREST RESEARCH

Keyword:  发根发根农杆菌发根诱导率;
Abstract: 研究了发根农杆菌的菌株类型、菌种浓度、浸染时间、预培养和共培养时间等对金银花发根诱导的影响.结果表明:农杆菌R1000培养OD600至0.6左右,稀释40倍浸染4 min时,诱导率最高,诱导率达58%.金银花外植体预培养5 d,与农杆菌共培养3 d,然后转入含500 mg/L羧苄青霉素的培养基杀菌培养是最适合的.
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