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Practical Study on Embryo Industrialization Production in Dairy Cows
Author(s): 
Pages: 27-29
Year: Issue:  6
Journal: CHINA DAIRY CATTLE

Keyword:  荷斯坦奶牛活体采卵显微操作性别鉴定胚胎移植胚胎工厂化生产;
Abstract: 本实验对20头荷斯坦青年奶牛进行活体采卵,共获卵母细胞131枚,回收率65.17%.以牛雄性特异序列BOV97M和牛属1.715卫星DNA序列设计引物进行PCR扩增,将发育至囊胚阶段的胚胎性别鉴定,共检测25枚胚胎,12枚为雌性,13枚为雄性,把雌性胚胎进行移植,怀孕3头,受胎率25%,产出母犊3头,准确率100%.
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