Author(s): GONG Zhen-cai, YANG Jun, TAN Xin
Pages: 57-57
Year: 2006 Issue:  3
Journal: SUBTROPICAL PLANT SCIENCE
Keyword:  苦马豆; 子叶; 胚轴; 组织培养;
Abstract: 以苦马豆种子在MS无植物生长调节剂的培养基上培养出无菌苗,以子叶和胚轴为外植体,在MS+6-BA2.0mg/L+2,4-D 0.5mg/L上诱导产生愈伤组织,在MS+6-BA 2.0mg/L+NAA 0.5mg/L培养基上继代培养,继代三次后在MS+6-BA2.0mg/L培养基上诱导产生丛芽,在MS+6-BA0.5mg/L培养基上进行幼苗培养,在幼苗长到3～4cm时移至1/2MS+IBA2.0mg/L培养基上生根.