Management of Real-time Fluorescence Quantitative PCR on Detection of Hepatitis B DNA
Author(s): SHEN Gang
Pages: 30-31,48
Year: 2015 Issue: 5
Journal: Hospital Management Forum
Keyword: PCR Real-time fluorescent quantitative PCR; Influencing factors;
Abstract: Objective To evaluate the accuracy of the hepatitis B DNA detection of real-time fluorescence quantitative PCR and to analyze the influencing factors, so as to put forward the management measures based on the influencing factors. Method From August 2011 to October 2014, 395 patients with diagnosed hepatitis B in our hospital were included in the study. The fasting blood was taken from cubital vein among 395 patients and real-time fluorescence quantitative PCR was conducted. We compared the results of the hepatitis B DNA detection of real-time fluorescence quantitative PCR with the results of clinical diagnosis to find out differences and analyzed the influencing factors of the differences. Results With the application of real-time fluorescence quantitative PCR, there were374 confirmed cases which accounted for 94.7%. Twenty one cases were missed diagnosis or misdiagnosis and accounted for 5.3%. There was no significant differences between the results of real-time fluorescence quantitative PCR and clinical diagnosis (P>0.05). The influencing factors of detection results included the conditions of the laboratory, operation of specimens (anticoagulation operation of specimens, specimen storage and the influence of hemolysis and lipid) and nucleic acid extraction methods. Conclusion The results of HBV-DNA detection of real-time fluorescent quantitative PCR have high accuracy rate and application value, but missed diagnosis or misdiagnosis also exist. Management of real-time fluorescent quantitative PCR should be strengthened in order to conduct integrated prevention in different aspects.
Pages: 30-31,48
Year: 2015 Issue: 5
Journal: Hospital Management Forum
Keyword: PCR Real-time fluorescent quantitative PCR; Influencing factors;
Abstract: Objective To evaluate the accuracy of the hepatitis B DNA detection of real-time fluorescence quantitative PCR and to analyze the influencing factors, so as to put forward the management measures based on the influencing factors. Method From August 2011 to October 2014, 395 patients with diagnosed hepatitis B in our hospital were included in the study. The fasting blood was taken from cubital vein among 395 patients and real-time fluorescence quantitative PCR was conducted. We compared the results of the hepatitis B DNA detection of real-time fluorescence quantitative PCR with the results of clinical diagnosis to find out differences and analyzed the influencing factors of the differences. Results With the application of real-time fluorescence quantitative PCR, there were374 confirmed cases which accounted for 94.7%. Twenty one cases were missed diagnosis or misdiagnosis and accounted for 5.3%. There was no significant differences between the results of real-time fluorescence quantitative PCR and clinical diagnosis (P>0.05). The influencing factors of detection results included the conditions of the laboratory, operation of specimens (anticoagulation operation of specimens, specimen storage and the influence of hemolysis and lipid) and nucleic acid extraction methods. Conclusion The results of HBV-DNA detection of real-time fluorescent quantitative PCR have high accuracy rate and application value, but missed diagnosis or misdiagnosis also exist. Management of real-time fluorescent quantitative PCR should be strengthened in order to conduct integrated prevention in different aspects.
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