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Identification of F1 hybrid progenies between Miscanthus sacchariflorus and M.lutarioriparius by SSR markers
Author(s): WEN Hao, AI Xin, JIANG Jian-xiong, ZHU Yu-ye, LONG Wei, YI Zi-li
Pages: 7-
11,22
Year: 2013
Issue:
4
Journal: Grassland and Turf
Keyword: Miscanthus sacchariflorus; M.lutarioriparius; hybrid identification; SSR;
Abstract: 选择来自芒属及近缘属的95对SSR引物对获与南获的F1杂交后代的真实性进行了鉴定.结果表明,共有6对SSR引物具有杂交种鉴别能力,其中,3对来自玉米,1对来自甘蔗,2对来自芒.选用特征带稳定清晰的2对双亲互补型引物对254个杂种后代单株进行检测,其中HAU-281鉴定出真杂交种250株,真杂种率为98.42%;HAU-438鉴定出真杂交种249株,真杂种率为98.03%,两引物检出率吻合度为99.60%.结果表明,SSR标记技术能有效应用于芒属种间杂种的真实性鉴定,为芒属植物杂交育种及遗传改良奠定了重要的基础.
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