The establishment and optimization of regeneration system of pepino stem-apex meristem in vitro
Author(s): ZHANG Fei-fei, ZHANG Jin-wen, SHI Jing
Pages: 34-38
Year: 2013 Issue: 4
Journal: Grassland and Turf
Keyword: pepino; stem-apex virus-free; regeneration system;
Abstract: 进行了建立并优化人参果茎尖分生组织试管苗的再生体系的研究.结果表明,对茎尖进行高温脱毒,脱毒率达90％,以组培脱毒苗叶片和茎段为材料,以MS为基础培养基,采用正交设计筛选出愈伤诱导及分化培养基分别为:1 mg/L 6-BA+1.6 mg/L ZT+0.2 mg/L 2,4-D和2 mg/L 6-BA+0.1mg/L IAA+1 mg/L GA3+2 mg/L ZT,适宜的生根培养基为:1/2 MS培养基+15 g/L蔗糖+0.5mg/L NAA.为人参果离体快繁及转基因研究奠定了基础.
Pages: 34-38
Year: 2013 Issue: 4
Journal: Grassland and Turf
Keyword: pepino; stem-apex virus-free; regeneration system;
Abstract: 进行了建立并优化人参果茎尖分生组织试管苗的再生体系的研究.结果表明,对茎尖进行高温脱毒,脱毒率达90％,以组培脱毒苗叶片和茎段为材料,以MS为基础培养基,采用正交设计筛选出愈伤诱导及分化培养基分别为:1 mg/L 6-BA+1.6 mg/L ZT+0.2 mg/L 2,4-D和2 mg/L 6-BA+0.1mg/L IAA+1 mg/L GA3+2 mg/L ZT,适宜的生根培养基为:1/2 MS培养基+15 g/L蔗糖+0.5mg/L NAA.为人参果离体快繁及转基因研究奠定了基础.
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