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Effects of Different Cut Chrysanthemum Culticars and Treatments on Callus Induction and Differentiation
Author(s): Shi Song, Li Qing, Zhao Shuang, Dai Silan, Li Nana
Pages: 77-
81
Year: 2013
Issue:
1
Journal: Journal of Northeast Forestry University
Keyword: Dendrathema morifolium; Callus; Induction; Plant growth regulators; Light treatment;
Abstract: 以切花菊‘神马’‘雪山’‘雪神’‘粉贵人’‘黄金’‘优香’6个品种的不同取材部位为试材,接种于含不同类型和质量浓度激素的培养基中,并在不同的光照条件下进行培养,研究影响切花菊愈伤组织诱导和分化的主要因素.结果表明:‘粉贵人’的叶片和茎段是最佳的外植体材料;在愈伤组织诱导试验中,2,4-D的效果优于NAA,6-BA的效果优于KT;在1 200 lx的光照条件下,愈伤组织诱导率高、增殖速度快,且后期分化能力强.试验还对愈伤组织不同的结构类型进行研究,认为Ⅱ、Ⅲ型愈伤组织,无论是从结构的疏松度、增殖速度还是后期分化能力都明显高于其他类型愈伤组织.
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