Effect of mastoparan-1 on lipopolysaccharide-induced acute hepatic injury in mice
Author(s): GUO Yi-bin, ZHENG Qing-yi, CHEN Jin-he, CAI Shao-fa, CAO Hong-wei, ZHENG Jiang, XIAO Guang-xia
Pages: 53-56
Year: 2009 Issue: 1
Journal: Chinese Journal of Burns
Keyword: Endotoxins; Hepatic injury; Inflammatory response; Mastoparan;
Abstract: Objective To observe the effect of mastoparan-1 (MP-1) on lipopolysaccharide (LPS)-induced acute hepatic injury in mice and probe into its possible mechanism. Methods One hun-dred and four BALB/c mice were randomly divided into healthy control group(n=8, without treatment, HC), LPS group (n=48, with injection of LPS 5 mg/kg via tail vein), and MP-1 group (n=48, with in-jection of LPS 5 mg/kg and MP-1 3 mg/kg via tail vein). Mice in LPS group and MP-1 group were sacri-ficed at 2nd, 6th, 12th, 24th, 48th and 72nd post injection hour (PIH), 8 mice at each time point in each group. Blood samples were collected for determination of plasma levels of LPS by kinetic turbidimetric limu-lus test, TNF-α and IL-6 by ELISA, serum levels of ALT and AST by automatic biochemistry analyzer re-spectively. Hepatic tissue samples were collected for determination of TLR4, TNF-α and IL-6 mRNA by re-al-time fluorescent quantitation reverse transcription polymerase chain reaction, along with the observation of pathological changes in hepatic tissue at each time point. Above-mentioned examinations were also performed in HC group. Results Compared with those of HC group, plasma levels of LPS and TNF-α in LPS group significantly increased at 2nd PIH(18 320.50±2782.50 EU/mL and 988±130 ng/L, respectively), then decreased gradually to 1.80±0.80 EU/mL and 150±44 ng/L at 72nd PIH, which was close to those of HC group. The values of IL-6, ALT and AST peaked at 12th PIH, which declined to the levels close to those of HC group at 72nd PIH. Meanwhile, the expressions of TLR4, TNF-α and IL-6 mRNA in liver were remark-ably up-regulated after injection, and the pathological changes in hepatic tissue pronounced significantly at 12nd, 24th and 48th PIH. Compared with those of LPS group, the levels of LPS, cytokines, ALT and AST de-creased in MP-1 group in different degrees after injection (P <0.05 or P <0. 01), genes expression(P < 0.05 or P <0.01) and pathological changes was respectively suppressed and alleviated in hepatic tissue. Conclusions MP-1 can alleviate LPS-induced acute hepatic injury in mice, which may be associated with its neutralization of LPS and attenuation of synthesis and release of inflammatory mediators.
Pages: 53-56
Year: 2009 Issue: 1
Journal: Chinese Journal of Burns
Keyword: Endotoxins; Hepatic injury; Inflammatory response; Mastoparan;
Abstract: Objective To observe the effect of mastoparan-1 (MP-1) on lipopolysaccharide (LPS)-induced acute hepatic injury in mice and probe into its possible mechanism. Methods One hun-dred and four BALB/c mice were randomly divided into healthy control group(n=8, without treatment, HC), LPS group (n=48, with injection of LPS 5 mg/kg via tail vein), and MP-1 group (n=48, with in-jection of LPS 5 mg/kg and MP-1 3 mg/kg via tail vein). Mice in LPS group and MP-1 group were sacri-ficed at 2nd, 6th, 12th, 24th, 48th and 72nd post injection hour (PIH), 8 mice at each time point in each group. Blood samples were collected for determination of plasma levels of LPS by kinetic turbidimetric limu-lus test, TNF-α and IL-6 by ELISA, serum levels of ALT and AST by automatic biochemistry analyzer re-spectively. Hepatic tissue samples were collected for determination of TLR4, TNF-α and IL-6 mRNA by re-al-time fluorescent quantitation reverse transcription polymerase chain reaction, along with the observation of pathological changes in hepatic tissue at each time point. Above-mentioned examinations were also performed in HC group. Results Compared with those of HC group, plasma levels of LPS and TNF-α in LPS group significantly increased at 2nd PIH(18 320.50±2782.50 EU/mL and 988±130 ng/L, respectively), then decreased gradually to 1.80±0.80 EU/mL and 150±44 ng/L at 72nd PIH, which was close to those of HC group. The values of IL-6, ALT and AST peaked at 12th PIH, which declined to the levels close to those of HC group at 72nd PIH. Meanwhile, the expressions of TLR4, TNF-α and IL-6 mRNA in liver were remark-ably up-regulated after injection, and the pathological changes in hepatic tissue pronounced significantly at 12nd, 24th and 48th PIH. Compared with those of LPS group, the levels of LPS, cytokines, ALT and AST de-creased in MP-1 group in different degrees after injection (P <0.05 or P <0. 01), genes expression(P < 0.05 or P <0.01) and pathological changes was respectively suppressed and alleviated in hepatic tissue. Conclusions MP-1 can alleviate LPS-induced acute hepatic injury in mice, which may be associated with its neutralization of LPS and attenuation of synthesis and release of inflammatory mediators.
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