Relationship between learning and memory ability and expression of hippocampal N-methyl-D-aspar-tic acid (NMDA) in burn rats with depression
Author(s): HE Mei, QIN Xia, YANG Guo-yu, FENG Zheng-zhi, YANG Zong-cheng
Pages: 46-48
Year: 2009 Issue: 1
Journal: Chinese Journal of Burns
Keyword: Burns; Depression; Memory disorders; N-Methylaspartate; Hippocampus;
Abstract: Objective To observe changes of learning and memory ability (LMA) in burn rats with depression, and study the relationship between LMA and expression of hippocampal NMDA. Methods According to simple random method, 46 Wistar rats were divided into burn group (B, with 30% TBSA deep partial-thickness burn, n=10), depression group (D, with moderate stress stimulation in chronic and unpre-dictable, n=12), B+D group (with the same stress stimulation inflicted to B group after burn, n=12), healthy control group(HC, without treatment, n=12). Changes in escape latency was examined in water maze test. Expression of hippocampal NMDA in CA1, CA2 regions and dentate gyrus were observed with im-munohistochemistry. Results Compared with that of HC group (22±20 s), water maze escape latency in B, D, B+D groups on 2 day after training prolonged (38±31, 41±36, 42±33 s, respectively, P <0.05 or P < 0. 01). Water maze escape latency in B+D group on 4th day after training was longer than that of oth-er groups(P <0. 01). There was no obvious difference in positive expression of NMDA in CA1, CA2 re-gions among groups (P>0.05). The positive count of NM DA in dentate gyms in D group (198±14) and B+D group(191±6) were lower than that of HC group (224±23, P <0.05 or P <0.01), but there was no obvious difference between HC group and B group (219±25, P > 0.05). Conclusions Burn compli-cated with depression can reduce LMA, which may be due to a decrease in NMDA in dentate gyrus.
Pages: 46-48
Year: 2009 Issue: 1
Journal: Chinese Journal of Burns
Keyword: Burns; Depression; Memory disorders; N-Methylaspartate; Hippocampus;
Abstract: Objective To observe changes of learning and memory ability (LMA) in burn rats with depression, and study the relationship between LMA and expression of hippocampal NMDA. Methods According to simple random method, 46 Wistar rats were divided into burn group (B, with 30% TBSA deep partial-thickness burn, n=10), depression group (D, with moderate stress stimulation in chronic and unpre-dictable, n=12), B+D group (with the same stress stimulation inflicted to B group after burn, n=12), healthy control group(HC, without treatment, n=12). Changes in escape latency was examined in water maze test. Expression of hippocampal NMDA in CA1, CA2 regions and dentate gyrus were observed with im-munohistochemistry. Results Compared with that of HC group (22±20 s), water maze escape latency in B, D, B+D groups on 2 day after training prolonged (38±31, 41±36, 42±33 s, respectively, P <0.05 or P < 0. 01). Water maze escape latency in B+D group on 4th day after training was longer than that of oth-er groups(P <0. 01). There was no obvious difference in positive expression of NMDA in CA1, CA2 re-gions among groups (P>0.05). The positive count of NM DA in dentate gyms in D group (198±14) and B+D group(191±6) were lower than that of HC group (224±23, P <0.05 or P <0.01), but there was no obvious difference between HC group and B group (219±25, P > 0.05). Conclusions Burn compli-cated with depression can reduce LMA, which may be due to a decrease in NMDA in dentate gyrus.
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