Effect of intensive insulin therapy on apoptosis-related ligands in serum in rats with severe scald
Author(s): DUAN Hong-jie, CHAI Jia-ke, SHENG Zhi-yong, YAO Yong-ming, YIN Hui-nan, SHEN Chuan-an, WU Yan-qiu, HU Quan, LIANG Li-ming
Pages: 42-45
Year: 2009 Issue: 1
Journal: Chinese Journal of Burns
Keyword: Burns; Insulin; TNF-related apoptosis-inducing ligand;
Abstract: Objective To investigate changes in apoptosis-related ligands in serum in rats with se-vere scald and the effect of intensive insulin therapy on the changes. Methods One hundred and fifty Wistar rats were randomly divided into 3 groups: sham burn(SB), scald(S) and treatment(T) groups. Rats in S and T groups were inflicted with 40% TBSA full-thickness burn, followed by intraperitoneal injection with 40 mL/kg of isotonic saline for resuscitation. Rats in T group were subcutaneously injected insulin in a dose of 0.25 U/100 g24 hours after burn injury, and every 12 hours for 5 days (0.25, 0.50, 0.75, 1.00, 1.25 U/100 g each day, respectively) to control the level of blood glucose between 3 and 6 mmol/L. Rats in SB group were sham scalded at 37 ℃ without resuscitation. Blood was drawn from abdominal aorta on 1, 4, 7, 10, 14 post burn day(PBD) for determination of serum levels of TNF-α, soluble Fas ligand(sFasL) and soluble Fas receptor(sFas) by enzyme-linked immunosorbent assay (ELISA), and insulin by radioim-munity assay (RIA). Results The serum level of TNF-α in S group peaked on 1 PBD(30.9±8.7) ng/L, which showed statistically significant difference when compared with that of SB and T groups (12.7±2.8) ng/L, (16.8±4.7)ng/L, respectively, P < 0.01), then lowered gradually to become similar to that of SB group on 7 PBD. The level of TNF-α in T group increased gradually, but was obviously lower than that of S group on 1, 4, 7 PBD(P<0.01). The level of sFasL in S (on 7-14 PBD) and T(4-10 PBD) groups was significantly higher than that in SB group (P <0.05), then lowered to normal level. The levels of sFas on 4-10 PBD in T group were obviously higher than that in S and SB group(P < 0.05). Ratio of sFasL to sFas in serum of S group was higher than that in SB group on 7, 10 PBD, which was higher than that in T group on 7 PBD(P <0.05). There was significant decrease in serum level of insulin in S group compared with that of SB group on 4-10 PBD(P<0.05). The level of insulin in T group increased on 1 PBD, peaked on 4 PBD(327±15 μU/mL), which was significantly higher than that in SB and S groups(42±15, 28±10 μU/mL, respectively, P <0.01) , then decreased gradually to normal level. Conclusions Insulin may inhibit apoptosis after burn by down-regulating secretion of apoptotic ligands.
Pages: 42-45
Year: 2009 Issue: 1
Journal: Chinese Journal of Burns
Keyword: Burns; Insulin; TNF-related apoptosis-inducing ligand;
Abstract: Objective To investigate changes in apoptosis-related ligands in serum in rats with se-vere scald and the effect of intensive insulin therapy on the changes. Methods One hundred and fifty Wistar rats were randomly divided into 3 groups: sham burn(SB), scald(S) and treatment(T) groups. Rats in S and T groups were inflicted with 40% TBSA full-thickness burn, followed by intraperitoneal injection with 40 mL/kg of isotonic saline for resuscitation. Rats in T group were subcutaneously injected insulin in a dose of 0.25 U/100 g24 hours after burn injury, and every 12 hours for 5 days (0.25, 0.50, 0.75, 1.00, 1.25 U/100 g each day, respectively) to control the level of blood glucose between 3 and 6 mmol/L. Rats in SB group were sham scalded at 37 ℃ without resuscitation. Blood was drawn from abdominal aorta on 1, 4, 7, 10, 14 post burn day(PBD) for determination of serum levels of TNF-α, soluble Fas ligand(sFasL) and soluble Fas receptor(sFas) by enzyme-linked immunosorbent assay (ELISA), and insulin by radioim-munity assay (RIA). Results The serum level of TNF-α in S group peaked on 1 PBD(30.9±8.7) ng/L, which showed statistically significant difference when compared with that of SB and T groups (12.7±2.8) ng/L, (16.8±4.7)ng/L, respectively, P < 0.01), then lowered gradually to become similar to that of SB group on 7 PBD. The level of TNF-α in T group increased gradually, but was obviously lower than that of S group on 1, 4, 7 PBD(P<0.01). The level of sFasL in S (on 7-14 PBD) and T(4-10 PBD) groups was significantly higher than that in SB group (P <0.05), then lowered to normal level. The levels of sFas on 4-10 PBD in T group were obviously higher than that in S and SB group(P < 0.05). Ratio of sFasL to sFas in serum of S group was higher than that in SB group on 7, 10 PBD, which was higher than that in T group on 7 PBD(P <0.05). There was significant decrease in serum level of insulin in S group compared with that of SB group on 4-10 PBD(P<0.05). The level of insulin in T group increased on 1 PBD, peaked on 4 PBD(327±15 μU/mL), which was significantly higher than that in SB and S groups(42±15, 28±10 μU/mL, respectively, P <0.01) , then decreased gradually to normal level. Conclusions Insulin may inhibit apoptosis after burn by down-regulating secretion of apoptotic ligands.
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