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Effects of phytohormones screening on callus induction of alfalfa with orthogonal design
Author(s): CHAI Yan-wen, MA Hui-ling, XIE Xiao-dong, LIU Yong-cai
Pages: 40-
43,47
Year: 2008
Issue:
5
Journal: GRASSLAND AND TURF
Keyword: 紫花苜蓿; 正交设计; 植物激素; 愈伤组织诱导;
Abstract: 以MS+2,4-D(2.0 mg/L)为基本培养基,附加NAA、6-BA和KT 3种植物激素,采用正交试验设计法研究不同浓度激素配比对和田苜蓿,秘鲁苜蓿和甘农3号苜蓿下胚轴愈伤组织诱导的影响.结果表明:对于秘鲁苜蓿和甘农3号苜蓿,最适愈伤组织诱导的培养基均为MS+2,4-D(2.0 mg/L)+6-BA(0.5 mg/L)+NAA(1.0 mg/L),各因素影响程度为6-BA>NAA>KT.对于和田苜蓿,最适愈伤组织诱导的培养基为MS+2,4-D(2.0 mg/L)+6-BA(1.0 mg/L)+KT(1.0 mg/L)+NAA(1.0mg/L),各因素影响程度为6-BA>KT>NAA.
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