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Issue:
Study on a New Complex System of Determination of Cysteine by Spectrofluorimetry
Author(s):
HUANG Zhi-biao
,
WANG Wei-sheng
Pages:
60
-
63
Year:
2008
Issue:
1
Journal:
GUANGXI SCIENCES
Keyword:
半胱氨酸
;
荧光光度法
;
In(Ⅲ)-8-羟基喹啉-5-磺酸荧光络合体系
;
Abstract:
在硼酸缓冲液和In(Ⅲ)-HQS荧光络合体系中加入一定浓度的半胱氨酸,体系的荧光强度与半胱氨酸的加入量成正比,从而提出一种新的荧光光度法测定半胱氨酸.该方法测定半胱氨酸的检出限为15ng·ml-1(SNR=3),大多数的氨基酸即使浓度较高时也无干扰.用该方法测定胱氨酸电解液中的半胱氨酸回收率为95.7%~101.3%.
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