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Content determination of prulixacin by HPLC
Pages: 594-596
Year: Issue:  10
Journal: CHINESE JOURNAL OF ANTIBIOTICS

Keyword:  普卢利沙星高效液相色谱含量测定;
Abstract: 目的建立普卢利沙星的含量测定方法.方法采用高效液相色谱法,以十八烷基键合硅胶柱(Kromasil ODS柱,4.6mm×250mm,5μm)为色谱柱,0.025mol/L的磷酸溶液(三乙胺调pH值2.5):甲醇(50:50)为流动相,流速1.0ml/min,检测波长278nm.结果普卢利沙星在20.03~100.15μg/ml浓度范围内,峰面积与浓度线性关系良好,r=0.9999.结论所建立方法快速、简捷、准确度高,专属性强,适宜于普卢利沙星的含量测定.
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