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In vitro study on specific immunity against prostate cancer induced by dendritic cells derived from fetal organs
Author(s): 
Pages: 695-698
Year: Issue:  10
Journal: CHINESE JOURNAL OF UROLOGY

Keyword:  胎儿树突细胞前列腺癌免疫治疗热休克蛋白;
Abstract: 目的研究胎儿来源树突状细胞(DC)体外诱导抗前列腺癌的特异性细胞免疫效果.方法从胎儿骨髓、肝脏获得单个核细胞,经重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)、重组人白细胞介素-4(rhIL-4)和重组人肿瘤坏死因子-α(rhTNF-α)诱导产生DC.50%~70%硫酸铵饱和沉淀法获取前列腺癌细胞DU145含热休克蛋白(HSP)成分的细胞溶解物,以该抗原负载DC,激活胎脾细胞产生肿瘤特异性细胞毒性T淋巴细胞(CTL).MTT法检测CTL对DU145、PC3和EJ细胞的杀伤效应.结果胎儿骨髓、肝脏可诱导出功能成熟的DC,高表达CDla、CD86、HLA-DR和CD83.负载DU145抗原的DC可诱导产生CD+8 CTL.CD+8T细胞表型由转化前的(14.09±2.46)%变为转化后的(62.76±2.64)%.对DU145细胞有明显细胞毒作用,显著高于对EJ细胞杀伤效应(P<0.01).结论含HSP成分的肿瘤细胞溶解物负载胎儿来源DC,体外可诱导出特异性抗肿瘤免疫应答.
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