The server is under maintenance between 08:00 to 12:00 (GMT+08:00), and please visit later.
We apologize for any inconvenience caused
Login  | Sign Up  |  Oriprobe Inc. Feed
China/Asia On Demand
Journal Articles
Laws/Policies/Regulations
Companies/Products
Expression of vitreoscilla hemoglobin gene(vgb) in E. coli
Author(s): 
Pages: 7-9
Year: Issue:  8
Journal: HEILONGJIANG ANIMAL SCIENCE AND VETERINARY MEDICINE

Keyword:  透明颤菌血红蛋白基因表达;
Abstract: 利用PCR技术将透明颤菌血红蛋白基因(vgb)克隆到融合表达载体pET28a,在大肠杆菌BL21(DE3)表达.重组蛋白在30 ℃诱导获得可溶表达.利用Ni2+亲和层析对重组蛋白进行了纯化,得到重组的透明颤菌血红蛋白,蛋白呈红色.实验现象显示重组蛋白与血红素相结合.紫外光区和可见光区波长扫描分析显示:蛋白粗提物和纯化后的血红蛋白在230 nm和413 nm都有强吸收峰,初步表明,尽管重组蛋白比天然蛋白多36个氨基酸,重组蛋白仍然具有生理活性.诱导后4 h和6 h的培养液氨基乙酰丙酸含量分别达到17 mg/L和21 mg/L,说明重组蛋白的表达明显促进了体内heme合成途径.
Related Articles
loading...