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Intracytoplasmic Sperm Injection Using Cryopreserved-thawed Testicular Spermatozoa with Testicular Fine Needle Aspiration
Author(s): 
Pages: 517-519
Year: Issue:  7
Journal: NATIONAL JOURNAL OF ANDROLOGY

Keyword:  非梗阻性无精子症经皮睾丸微穿刺活检精子冷冻保存卵胞质内单精子注射;
Abstract: 目的:对经皮睾丸微穿刺活检后冷冻保存精子卵胞质内单精子注射(ICSI)治疗非梗阻性无精子症所致不育进行临床总结,并对其影响治疗结果的因素进行探讨.方法:对62例非梗阻性无精子症患者进行经皮睾丸微穿刺活检,发现活动精子者(35例)对睾丸活检组织进行冷冻保存;女方促排卵常规使用促性腺激素释放激动剂(GnRHa)/卵泡刺激素(FSH)/人绒毛膜促性腺激素(hCG)方案,B超监测卵泡发育情况并引导经阴道取卵,冷冻的睾丸组织解冻后行ICSI,良好胚胎进行移植.结果:取卵周期为35个,冷冻的睾丸精子解冻后行ICSI,35个周期进行常规胚胎移植.13例临床妊娠.启动周期、取卵周期与移植周期临床妊娠率均为37.14%(13/35).结论:经皮睾丸微穿刺活检后ICSI是治疗非梗阻性无精子症所致不育的最主要和有效的方法;活检后对有活动精子的睾丸组织进行冷冻保存不影响治疗结果,可以减少患者睾丸活检的次数,减轻患者痛苦.
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