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Isolation, Purification and Identification of Geniposidic Acid in Eucommia ulmoides Oliv. By Silica Column Chromatography, Reversed-Phase High Performance Liquid Chromatography, Liquid Chromatography-Electrospray Ionization Mass Spectrometry and Nuclear Magnetic Resonance
Author(s): 
Pages: 534-537
Year: Issue:  5
Journal: CHINESE JOURNAL OF CHROMATOGRAPHY

Keyword:  柱色谱法反相高效液相色谱法液相色谱-电喷雾质谱核磁共振京尼平甙酸杜仲中药;
Abstract: 建立了杜仲中京尼平甙酸的硅胶柱色谱分离纯化及反相高效液相色谱/液相色谱-电喷雾质谱/核磁共振(RP-HPLC/LC-ESI-MS/NMR)鉴定方法.杜仲皮经粉碎后,用70%乙醇提取,提取液经减压蒸馏至干,再用甲醇溶解,然后吸附于硅胶上,并以不同体积比的氯仿-甲醇混合液按洗脱剂的极性由小到大进行洗脱,以RP-HPLC法检测洗脱液中物质的种类和含量.结果表明:洗脱剂为氯仿-甲醇(体积比为8:1)的洗脱液经分析为单一组分,其保留时间为5.142 min;以对照品京尼平甙酸添加法测定,其峰高增加;相关紫外光谱和红外光谱检测结果与京尼平甙酸对照品基本一致;结合LC-ESI-MS、1H-NMR和13C-NMR等测定,确定该洗脱组分为京尼平甙酸.
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