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Simultaneous Determination of Water-Soluble Vitamins C, B1, B2 and B6 in Almonds by High Performance Liquid Chromatography
Author(s): 
Pages: 538-541
Year: Issue:  5
Journal: CHINESE JOURNAL OF CHROMATOGRAPHY

Keyword:  高效液相色谱水溶性维生素扁桃仁;
Abstract: 建立了用高效液相色谱同时测定扁桃仁中4种水溶性维生素C,B1,B2和B6的方法.以酸水解法处理样品,在Inertsil ODS-3柱(25 cm×4.6 mm i.d., 5.0 μm)上,以0.05 mol/L KH2PO4 (pH 6.0)-甲醇(体积比为70:30)为流动相进行等度洗脱,检测波长265 nm条件下,对陕西蒲城扁桃仁中的水溶性维生素C,B1,B2和B6的含量进行了同时测定.4种水溶性维生素在其质量浓度为5.0~50 mg/L时线性良好(r=0.999 0~0.999 7);添加水平为5.0~20.0 mg/kg时,平均回收率为91.77% ~99.30% ,相对标准偏差(n=3)为0.31% ~1.98% .测得陕西蒲城产扁桃仁中维生素B2的含量为4.27~4.53 mg/kg,维生素B1的含量为0.799~0.838 mg/kg,未检出维生素C和维生素B6.该法简便、快速,准确性和重现性均较好,对果仁中水溶性维生素的测定具有一定的参考价值.
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