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Simultaneous Determination of Sudan Red Dyes in Foods by High Performance Liquid Chromatography with a Clean-Up Procedure by Gel Column
Author(s): 
Pages: 542-544
Year: Issue:  5
Journal: CHINESE JOURNAL OF CHROMATOGRAPHY

Keyword:  凝胶柱高效液相色谱苏丹红食品;
Abstract: 建立了凝胶柱净化-高效液相色谱同时检测食品中苏丹红Ⅰ,Ⅱ,Ⅲ和Ⅳ的方法.样品用乙醇提取,提取液经Bio-Beads SX3凝胶柱(200 mm×10 mm i.d.)净化,用环己烷-乙酸乙酯(体积比为1:1)洗脱.采用Symmetry Shield RP18柱(250 mm×4.6 mm i.d., 5 μm)分离,以100%甲醇为流动相,流速1.5 mL/min;用二极管阵列检测器检测,检测波长478 nm.上述4种苏丹红组分在其质量浓度为0.1~10.0 mg/L时有良好的线性关系(r>0.999),方法的检测限为7~14 μg/kg;平均加标回收率为80.7% ~96.3% (添加水平为0.25,2.5 mg/kg),相对标准偏差为2.4% ~5.9% .方法灵敏可靠,能满足食品中苏丹红检测的需要.
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