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The effect of matrix metalloproteinase 2 on interstitium of rat myocardium with ischemic reperfusion
Author(s): 
Pages: 452-455
Year: Issue:  5
Journal: THE JOURNAL OF PRACTICAL MEDICINE

Keyword:  基质金属蛋白酶缺血再灌注损伤心肌大鼠;
Abstract: 目的:探讨基质金属蛋白酶-2( MMP-2)在鼠缺血再灌注心肌的表达,及其与心肌间质损伤和心功能变化的关系.方法: Sprague-Dawley(SD)大鼠 24只,随机分为对照组、缺血再灌注组和强力霉素组,每组 8只.分别建立改良的 Langendorrf离体心灌注模型,测定左室收缩峰压及压力微分,氯胺 T法测定胶原含量,免疫组化法测定Ⅰ /Ⅲ胶原比例,酶谱法测定冠脉回流液不同时间点的 MMP-2及其酶原( pro-MMP-2). Western blot检测心肌MMP-2的表达.结果:MMP-2活性在心肌缺血再灌注早期即明显升高,且其活性与心功能的恢复呈明显的相关性.心肌胶原含量和Ⅰ /Ⅲ比值较对照组均明显下降.强力霉素组表现出明显的保护作用.结论:MMP-2在心肌缺血再灌注间质损伤中起关键作用,应用 MMP-2抑制剂可能成为保护心肌缺血再灌注损伤的新方法.
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