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A Method of Total RNA Isolation in Lotus Tissues
Pages: 475-477
Year: Issue:  5
Journal: JOURNAL OF WUHAN BOTANICAL RESEARCH

Keyword:  莲藕组织RNA提取多糖酚类物质;
Abstract: 莲藕组织富含多糖、脂质、酚类等物质,用一般的方法较难提取高质量的RNA.在改进前人方法的基础上,建立了一种高效、简单的CTAB-LiCl提取法,能快速提取高质量的莲藕组织总RNA,并且产率高、完整性好、纯度高,能进一步满足RT-PCR等分子生物学实验的需要.此外,该方法也适用于其它富含多糖、脂质、酚类等物质的植物组织总RNA的提取.
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