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Expression and Activity Detection of the Catalytic Alpha Subunit of CK2 from Oryza sativa
Author(s): 
Pages: 544-548
Year: Issue:  4
Journal: JOURNAL OF FUDAN UNIVERSITY(NATURAL SCIENCE)

Keyword:  水稻蛋白质激酶CK2表达纯化活性测定;
Abstract: 用RT-PCR的方法分离到了水稻蛋白质激酶CK2催化亚基(OsCK2α)的cDNA,通过大肠杆菌高效表达系统表达了OsCK2α,并利用亲合层析的方法纯化了重组蛋白质GST-OsCK2α.得到的大肠杆菌重组蛋白质GST-OsCK2α能够体外磷酸化CK2的常用底物酪蛋白,并且该磷酸化反应能够被CK2的特异性抑制剂肝素所抑制,研究表明重组CK2α具有典型的蛋白质激酶CK2的活性,为研究水稻CK2及其底物的功能奠定了基础.
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