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Optimization of RAPD Reaction System for Cabbage
Author(s): LIU Chong, BO Tian-yue, GE Cai-lin, REN Yun-ying, CHEN Jin-xiu, YANG Xiao-feng
Pages: 114-
117
Year: 2005
Issue:
3
Journal: ACTA AGRICULTURAE SHANGHAI
Keyword: 甘蓝; RAPD; 体系优化;
Abstract: 以甘蓝(Brassica oleracea var.capitata)基因组DNA为模板,比较筛选RAPD扩增体系的各影响因素,优化了甘蓝的RAPD反应体系,调整了扩增程序.该体系反应总体积为20μL,其中25mmol/L MgCl2 2.0μL,10×PCR Buffer 2.0μL,10mmol/L dNTP 0.5μL,5U/μL Taq E 0.5μL,20ng/μL Primer 1.5μL,10ng/μL模板DNA 3μL,灭菌双蒸水10.5μL.适宜的扩增程序为:94℃预变性5min;94℃变性1min,37℃复性1min,72℃延伸1.5min,40个循环;72℃延伸10min后15℃保存.
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