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Isolation of High - quality RNA from Rat Brain Tissue
Author(s): 
Pages: 511-512
Year: Issue:  3
Journal: PRACTICAL PREVENTIVE MEDICINE

Keyword:  大鼠脑组织RNA提取Trizol方法学;
Abstract: 目的为探諸rizol试剂提取大鼠脑组织高质量RNA的最适取样量范围.方法在1mLTrizol试剂中分别加入100,50,25,12.5mg大鼠脑组织,提取其总RNA.结果每毫升Trizol中加入组织12.5mg时,提取的RNA完整性最好,纯度亦高.结论从大鼠脑组织中提取到高质量RNA,每毫升Trizol中加入脑组织最适取样量范围为10~20mg.
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