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Expression of human OX40L in BL21 E.coli and studies on its biological function
Author(s): 
Pages: 163-167,171
Year: Issue:  3
Journal: CHINESE JOURNAL OF IMMUNOLOGY

Keyword:  OX40LBL-21大肠杆菌表达生物学功能;
Abstract: 目的:克隆和表达人可溶性OX40L重组蛋白(sOX40L),探讨OX40L信号在T细胞对乳腺癌细胞反应中的协同刺激效应.方法:运用基因工程技术在大肠杆菌中表达人重组融合蛋白GST-sOX40L,Western blot分析表达蛋白的特异性;采用体外T细胞和乳腺癌细胞混合培养体系,观察对T细胞生长和增殖的影响.结果:构建了GST-sOX40L原核表达体系,从而进行重组蛋白的有效表达;纯化的重组蛋白通过免疫印迹分析能被特异性单抗识别,并在体外培养体系中显示出协同刺激T细胞对乳腺肿瘤细胞的反应性:促进T细胞生长、增殖以及IL-2的分泌.结论:成功地研制了具有生物学活性的sOX40L,为体外激发肿瘤特异性T细胞提供良好的物质基础.
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