Author(s): LI Xiaoliu, CHEN Chao, LI Yanmei, SHI Hongling, WANG Hongmei
Pages: 23-26
Year: 2005 Issue:  1
Journal: PLANT PHYSIOLOGY COMMUNICATIONS
Keyword:  除虫菊; 细胞悬浮培养; 生长条件;
Abstract: 除虫菊悬浮细胞从培养后第8天开始迅速生长,14 d达到最大生长量,其最适培养基为:MS+4mg·L-12,4-D+0.1mg·L-1NAA+0.3 mg·L-16-BA+30 g·L-1蔗糖,最适接种量为15 g·L-1,摇瓶装液的体积分数为40%,初始培养的最适pH值为5.8,收获时pH下降为4.6±1,最适培养温度为25℃.