Transdifferentiation of bone marrow stromal cells into neurons in vitro
Author(s): ZHANG Jun, SHAO Ming, GONG Dongmei, ZHANG Xinying, DONG Deli, YANG Baofeng
Pages: 4886-4887
Year: 2004 Issue: 23
Journal: CHINESE JOURNAL OF CLINICAL REHABILITATION
Keyword: 骨髓细胞; 细胞培养; 神经元; 细胞系; 转化;
Abstract: BACKGROUND: As discovered by the researches, bone marrow stromal cells(BMSCs) could travel to different parts of the body after transplantation, proliferate and transdifferentiate into corresponding cells to recover to some extent the functions of injured organs. It has been proved that BMSCs could transdifferentiate into mesoblast and endoblast cells in vitro.OBJECTIVE: To investigate the possibility of the transdifferentiation of BMSCs into neurons.DESIGN: A controlled repeated measurement design was conducted.SETTING and PARTICIPANTS: Study was conducted in the Department of Orthopaedics, the First Affiliated Hospital of Harbin Medical University. Subjects were healthy male SD rats with a body mass from 120 g to 150 g in clean grade obtained from the Laboratory Animal Department, the First Affiliated Hospital of Harbin Medical University.INTERVENTIONS: Three different schemes were used to induce the BMSCs after primary and passage culture. General observation, immunocytochemistry and electrophysiology examinations as well as long-term culture study were conducted.MAIN OUTCOME MEASURES: The results of BMSC induction, immunocytochemistry and electrophysiology.RESULTS: One hour after adding inducer in the first induction scheme, there were 50% of BMSCs transdifferentiating into neurons and 70% - 80%into neurons at 90 minutes. At 24 hours after adding inducer in the second induction scheme, there were 10% of BMSCs transdifferentiating into neurons. 40% of BMSCs were adding in the third scheme, there were 90% of BMSCs transdifferentiating into neurons at 24 hours with small soma and short synapse; when 80% of BMSCs were adding, 40% of the transdifferetiated neurons were in a nest arrangement. BMSCs could be transdifferentiated into neurons with clear synapse(induction rate ＞ 80% ), which expressed specific markers of neuron, neuro-specific enolase and glial fibrillary acidic protein, and also had the current characteristics of the early neurons at the same time.CONCLUSION: It is possible that BMSCs can be induced into early neurons.
Pages: 4886-4887
Year: 2004 Issue: 23
Journal: CHINESE JOURNAL OF CLINICAL REHABILITATION
Keyword: 骨髓细胞; 细胞培养; 神经元; 细胞系; 转化;
Abstract: BACKGROUND: As discovered by the researches, bone marrow stromal cells(BMSCs) could travel to different parts of the body after transplantation, proliferate and transdifferentiate into corresponding cells to recover to some extent the functions of injured organs. It has been proved that BMSCs could transdifferentiate into mesoblast and endoblast cells in vitro.OBJECTIVE: To investigate the possibility of the transdifferentiation of BMSCs into neurons.DESIGN: A controlled repeated measurement design was conducted.SETTING and PARTICIPANTS: Study was conducted in the Department of Orthopaedics, the First Affiliated Hospital of Harbin Medical University. Subjects were healthy male SD rats with a body mass from 120 g to 150 g in clean grade obtained from the Laboratory Animal Department, the First Affiliated Hospital of Harbin Medical University.INTERVENTIONS: Three different schemes were used to induce the BMSCs after primary and passage culture. General observation, immunocytochemistry and electrophysiology examinations as well as long-term culture study were conducted.MAIN OUTCOME MEASURES: The results of BMSC induction, immunocytochemistry and electrophysiology.RESULTS: One hour after adding inducer in the first induction scheme, there were 50% of BMSCs transdifferentiating into neurons and 70% - 80%into neurons at 90 minutes. At 24 hours after adding inducer in the second induction scheme, there were 10% of BMSCs transdifferentiating into neurons. 40% of BMSCs were adding in the third scheme, there were 90% of BMSCs transdifferentiating into neurons at 24 hours with small soma and short synapse; when 80% of BMSCs were adding, 40% of the transdifferetiated neurons were in a nest arrangement. BMSCs could be transdifferentiated into neurons with clear synapse(induction rate ＞ 80% ), which expressed specific markers of neuron, neuro-specific enolase and glial fibrillary acidic protein, and also had the current characteristics of the early neurons at the same time.CONCLUSION: It is possible that BMSCs can be induced into early neurons.
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