Experimental study on the treatment of cerebral ischemia reper-fusion injury with magnesium sulfate
Author(s): PANG Zhong, ZHANG Weixi, WANG Xin
Pages: 2478-2479
Year: 2004 Issue: 13
Journal: CHINESE JOURNAL OF CLINICAL REHABILITATION
Keyword: 脑缺血; 再灌注损伤; 硫酸镁; 大鼠;
Abstract: 目的:探讨硫酸镁对大鼠局灶性脑缺血再灌注损伤后神经功能的保护作用.方法:制备易卒中型肾血管性高血压大鼠(stroke-pronereno vascular hy-pertension rats,RHRSP),用线栓法复制大脑中动脉阻塞(middle cerebralartery occlusion,MCAO)模型.实验1:造成缺血6 h再灌注18 h,治疗组于不同时程使用硫酸镁,测定脑梗死体积及脑水肿的变化.实验2:造成缺血2 h再灌1,3,5 d,治疗组于再灌注后立即使用硫酸镁,采用TUNEL法原位标记DNA片段,检测TUNEL阳性细胞的变化.结果:治疗组的梗死灶体积及其占全大脑体积百分比、两半球体积差值档显著减小,TUNEL阳性细胞亦明显减少(P＜0.05).结论:硫酸镁能减轻脑缺血再灌注损伤,早期用药效果更好,减少再灌注后细胞损伤很可能是其作用机制之一.
Pages: 2478-2479
Year: 2004 Issue: 13
Journal: CHINESE JOURNAL OF CLINICAL REHABILITATION
Keyword: 脑缺血; 再灌注损伤; 硫酸镁; 大鼠;
Abstract: 目的:探讨硫酸镁对大鼠局灶性脑缺血再灌注损伤后神经功能的保护作用.方法:制备易卒中型肾血管性高血压大鼠(stroke-pronereno vascular hy-pertension rats,RHRSP),用线栓法复制大脑中动脉阻塞(middle cerebralartery occlusion,MCAO)模型.实验1:造成缺血6 h再灌注18 h,治疗组于不同时程使用硫酸镁,测定脑梗死体积及脑水肿的变化.实验2:造成缺血2 h再灌1,3,5 d,治疗组于再灌注后立即使用硫酸镁,采用TUNEL法原位标记DNA片段,检测TUNEL阳性细胞的变化.结果:治疗组的梗死灶体积及其占全大脑体积百分比、两半球体积差值档显著减小,TUNEL阳性细胞亦明显减少(P＜0.05).结论:硫酸镁能减轻脑缺血再灌注损伤,早期用药效果更好,减少再灌注后细胞损伤很可能是其作用机制之一.
Citations
- Protective effects of ginkgo biloba extract on rats during cerebral ischemia/reperfusion Hu B Chinese Medical Journal
- 胰岛素对大鼠脑缺血再灌注时神经元凋亡及其相应基因的调控 于恺 中国临床康复
- Effects of propofol,midazolam and thiopental sodium on outcome and amino acids accumulation in focal cerebral ischemia-reperfusion in rats Chen LH Chinese Medical Journal
- Pretreatment with repeated electroacupuncture attenuates transient focal cerebral ischemic injury in rats Xiong ZL Chinese Medical Journal
- 大鼠脑缺血再灌注时脑水肿的实验研究 兰希发 中国临床康复
- A comparison of the accuracy and safety of vertebral body pin placement using a fluoroscopically guided versus an open surgical approach:an in vitro study Wheeler JL Veterinary Surgery
- 脑缺血再灌注对大鼠不同组织细胞凋亡及凋亡调控基因表达的影响 卓安山 中国临床康复
- Biochemical and morphological changes in herniated human intervertebral disc Ahsan R Journal of Orthopaedic Science
Related Articles