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Biocompatibility of self- processed deproteinized bone with marrow stromal cells cultured in vitro
Author(s): 
Pages: 1265-1268
Year: Issue:  11
Journal: CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA

Keyword:  脱蛋白骨组织工程骨髓基质细胞;
Abstract: 目的探讨表面脱钙的脱蛋白异体骨与骨髓基质细胞的生物相容性,为骨组织工程提供合适的支架材料.方法用经物理与化学方法处理而制得的脱蛋白骨与骨髓基质细胞(MSCs)复合培养,进行形态学观察、细胞增殖、ALP的活性、骨钙素的分泌与蛋白质含量检测;扫描电镜观察细胞在脱蛋白骨材料上的情况.结果骨髓基质细胞能在脱蛋白骨上贴附、增殖,其生长及功能不受影响.结论本方法所制的脱蛋白骨与MSCs具有良好的生物相容性,可作为MSCs的载体应用于骨组织工程.
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