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Antigenicity identification and gene immunization of SARS-coronavirus nucleocapsid protein
Author(s): 
Pages: 591-594
Year: Issue:  8
Journal: CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY

Keyword:  SARS冠状病毒核衣壳蛋白抗原性基因疫苗;
Abstract: 目的了解SARS冠状病毒(SARS-CoV)核衣壳蛋白(N蛋白)的抗原性及其基因疫苗的免疫原性.方法用大肠杆菌表达SARS-CoV的N蛋白,用SARS患者恢复期血清对其进行抗原性鉴定.再构建N蛋白基因疫苗,肌肉注射接种小鼠,检测小鼠血清中的抗N蛋白IgG抗体、脾细胞增殖和迟发型超敏反应.结果大肠杆菌重组表达的SARS-CoV N蛋白具有强抗原性,其基因疫苗能在小鼠有效诱导N蛋白特异性抗体和CD4+、CD8+ T淋巴细胞免疫应答.结论 SARS-CoV的N蛋白可作为重要的SARS血清学诊断抗原,并对于SARS的特异性预防和治疗有潜在的应用价值.
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