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Establishment of animal model for in vitro spermatogenesis by heterotopic grafting of neonatal mouse testis
Author(s): 
Pages: 591-594
Year: Issue:  6
Journal: JOURNAL OF PEKING UNIVERSITY(HEALTH SCIENCES)

Keyword:  睾丸组织移植精母细胞睾丸特异蛋白激酶小鼠;
Abstract: 目的:采用免疫缺陷小鼠作为孵化器,建立小鼠睾丸组织异体异位移植生成精子的实验模型.方法:以新生小鼠睾丸组织为供体,免疫缺陷小鼠为受体进行组织移植;采用反转录-聚合酶链反应(reverse transcriptase polymerase chain reaction,RT-PCR)对移植物中睾丸特异蛋白激酶(testis-specific protein kinase 1,TESK1)mRNA表达进行检测,并应用组织形态学分析法对生精细胞的发育作进一步的验证.结果:分子生物学及组织学观察显示,移植物组织不仅可像正常小鼠睾丸组织一样表达TESK1 mRNA,并且富含与正常成年小鼠睾丸组织中类似的精曲小管结构以及各级生精细胞和精子.结论:移植后的生精细胞可在异体异位继续发育生长,完成整个生精过程,最终发育精曲小管及成熟精子.
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