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Expression of OPG and RANKL at protein level in human periodontal ligament cells and the effect of 1 α,25 (OH) 2 vitamin D3 on the secretion of OPG protein in vitro
Author(s): 
Pages: 646-649
Year: Issue:  6
Journal: JOURNAL OF PEKING UNIVERSITY(HEALTH SCIENCES)

Keyword:  牙周组织细胞培养的骨化三醇膜糖蛋白类破骨细胞;
Abstract: 目的:研究人牙周膜细胞骨保护因子(osteoprotegerin,OPG)和破骨细胞分化因子(receptor activator nuclear factor kappa B ligand,RANKL)蛋白的表达,以及骨吸收促进因子1α,25(OH)2维生素D3对OPG蛋白表达的影响.方法:用系列酶消化法体外培养人牙周膜细胞,用免疫细胞化学检测细胞上表达的RANKL蛋白,以酶联免疫吸附实验检测无外源性刺激以及1α,25(OH)2维生素D3诱导2、4、6 d时细胞分泌到培养基中的OPG蛋白.结果:人牙周膜细胞胞膜和胞浆上表达RANKL蛋白,分泌OPG蛋白到培养基中.1α,25(OH)2维生素D3降低OPG蛋白的分泌.结论:人牙周膜细胞表达OPG和RANKL,1α,25(OH)2维生素D3影响OPG的表达,推测其通过OPG/RANKL通路参与骨改建.这一结论为进一步研究牙周膜细胞参与骨改建的机制打下基础.
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