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Comparative Quantification of ESR mRNA Levels in Several Tissues in Pigs
Author(s): LI Fenge, XIONG Yuanzhu, LEI Minggang, DENG Changyan, ZHENG Zuo, JIANG Siwen
Pages: 492-
494
Year: 2004
Issue:
5
Journal: JOURNAL OF HUAZHONG AGRICULTURAL UNIVERSITY
Keyword: 猪; ESR基因; 半定量RT-PCR; 表达;
Abstract: 以看家基因GAPDH为内标,采用半定量RT-PCR法检查了ESR mRNA在卵巢等12种组织中表达情况.结果表明ESR基因有2种表达产物,其中在肠、卵巢、脾、心、肺、肝等组织的表达产物为一种形式,而子宫、肌肉等组织为另一种形式,在肾中2种形式的表达产物共存.通过计算机凝胶成像分析系统,对胶进行扫描,显示出在12种组织中,ESR mRNA表达量在肺中最高,其次是卵巢、肠、心、子宫、脾、肝、肌肉等.在输卵管、肾、脂肪和乳腺组织中,ESR mRNA表达量很少.
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