Author(s): WEI Zhiwen, ZUO Keming, ZHANG Zuo, HE Wenyan, AN Jiankang, GAO Libo, CHEN Yuehua, WU Yanbin, NIU Jianguo
Pages: 124-126
Year: 2005 Issue:  2
Journal: JOURNAL OF FORENSIC MEDICINE
Keyword:  利多卡因; 气相色谱-质谱; 血液; 脑脊液;
Abstract: 目的建立血液和脑脊液中利多卡因的气相色谱-质谱联用定性、定量检测方法.方法血液或脑脊液盐酸酸化后,氢氧化钠碱化(pH=9),乙醚提取,氮气流下挥干,乙醇定容,气相色谱-质谱联用仪分析,选择离子监测模式检测(86,58,72,87),定性、定量检测血液和脑脊液中利多卡因.结果血液和脑脊液中利多卡因的线性范围为1.0～60.0μg·mL-1(r=0.9999),检出限为0.02μg·mL-1(S/N=3),加样回收率为85%～103%,麻醉致死犬血液和脑脊液中检出利多卡因,结果满意.结论该法选择性好,干扰少,灵敏,准确,可用于生物体液中利多卡因的定性和定量检测.