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A study of genetic polymorphism of the STR locus D7S2846、D19S400 and D18S535 in Hah population in Wenzhou
Author(s): ZHANG Hongqin, WU Shuzhen, BI Yuntian
Pages: 160-
161
Year: 2004
Issue:
3
Journal: JOURNAL OF FORENSIC MEDICINE
Keyword: 短串联重复序列; 聚合酶链反应; 遗传多态性;
Abstract: 目的研究D7S2846、D19S400和D18S535位点在温州汉族人群的遗传多态性.方法 EDTA抗凝血样采自194名无血缘关系温州地区汉族个体,用chelex-100法提取DNA,PCR扩增,聚丙烯酰胺凝胶电泳,银染显色分析.结果 D7S2846观察到6个等位基因及15种基因型;D19S400观察到10个等位基因及36种基因型;D18S535观察到8个等位基因及26种基因型.各基因座的杂合度(H)分别为:0.644、0.724、0.772;个人识别能力(Dp):0.854、0.940、0.938.结论三个STR基因座具有较高杂合度,等位基因分布符合Hardy-Weinberg平衡,在法医学应用和群体遗传学研究中有较高的价值.
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