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Tutin-induced epileptiform discharge of CA1 pyramidal cells in rat hippocampal slices
Author(s): 
Pages: 341-346
Year: Issue:  3
Journal: ACTA PHYSIOLOGICA SINICA

Keyword:  癫痫羟基马桑毒素海马脑片痫样放电NMDA受体非NMDA受体;
Abstract: 本研究采用离体海马脑片电生理研究技术,细胞外记录海马锥体细胞群体锋电位(population spike,PS),观察羟基马桑毒素(tutin)对大鼠海马脑片CA1区锥体细胞电活动的影响,探讨tutin是否具有致痫作用及其致痫机制.结果如下:(1)用40、30和20μg/ml浓度的turin灌流海马脑片,可显著增高由顺向刺激Schaffer侧支所诱发的PS的幅度,灌流tutin 30min时,PS第一个波的幅度分别为对照的(388.7±20.1)%、(317.2±19.1)%和(180.9±11.6)%(各组n=5,P<0.05).(2)伴随PS波幅的增高,可出现成串痫样放电波,波数4~11个不等.(3)灌流tutin后的部分脑片(n=9/34),在未刺激Schaffer侧支时也出现自发的成串、高幅痫样放电.(4)灌流CNQX阻断非NMDA受体后,再灌流tutin,PS幅度和放电波数均无显著性变化,即CNQX可完全抑制turin所致的痫样放电;灌流AP-5阻断NMDA受体后,tutin仍可使PS幅度增高但放电波数无显著性增加,即AP-5可部分抑制turin所致的痫样放电.上述结果表明,turin可使海马脑片锥体细胞兴奋活动增强,具有致痫作用;兴奋性谷氨酸受体尤其是非NMDA受体可能介导tutin的致痫作用.
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