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Possible involvement of caveolin-1 in the inhibition of endothelin-1 induced proliferation of vascular smooth muscle cells by 17β-estradiol
Author(s): 
Pages: 379-383
Year: Issue:  3
Journal: ACTA PHYSIOLOGICA SINICA

Keyword:  caveolin-1蛋白细胞质膜微囊内皮素血管平滑肌细胞17β-雌二醇;
Abstract: 本工作旨在研究Caveolin-1在17β-雌二醇(17β-estradiol,E2)抑制内皮素-1(endothelin-1,ET-1)诱导血管平滑肌细胞(vascular smooth muscle cells,VSMCs)增殖中的作用.在培养的VSMCs上,使用[3H]Thymidine([3H]TdR)掺入法、荧光免疫组化方法和Western blot观察E2处理VSMCs前后ET-1对DNA合成和caveolin-1蛋白表达的影响.结果显示,ET-1可以刺激VSMCs增殖.E2作用24 h后,可明显抑制ET-1的上述作用.免疫荧光证实,在VSMCs上有caveolin-1分布,ET-1刺激VSMCs增殖的过程中,VSMCs上caveolin-1蛋白荧光强度下降,而事先给予E2可逆转这种下降.Western blot证实,ET-1可抑制caveolin-1蛋白的表达而E2则可增加caveolin-1蛋白的表达.以上结果表明E2抑制ET-l诱导的VSMCs增殖可能与其增加caveolin-1蛋白表达有关.
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