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Cloning of Bidirectional pcbAB-pcbC Promoter Region from Cephalosporium acremonium and Its Application
Author(s): 
Pages: 255-257
Year: Issue:  2
Journal: ACTA MICROBIOLOGICA SINICA

Keyword:  顶头孢霉pcbAB-pcbC双向启动子区域转化;
Abstract: 用PCR方法从丝状真菌顶头孢霉中克隆出全长1.3kb的pcbAB-pcbC双向启动子DNA片段,通过转化子对博莱霉素的抗性证明了该启动子在顶头孢霉中的双向启动功能.另外,利用所克隆的pcbAB-pcbC双向启动子构建了一个用于顶头孢霉转化的质粒pYG13,并成功地将该质粒转化入顶头孢霉.pYG13含有博莱霉素抗性基因和透明颤菌血红蛋白基因(vgb), Southern杂交和CO结合实验分析显示vgb整合到顶头孢霉的基因组DNA中并表达了有活性的透明颤菌血红蛋白.
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